Question 2
1 ptWhat determines the optimal annealing temperature for PCR?
Heredity · Division B · Dodgen-Walton - GA · 2026
49 questions · 55 points · 50 min· 15 questions from the original omitted
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Materials allowed: One (1) sheet of 8.5”x11” sheet of paper that may contain information on both sides in any form and from any source. The sheet of paper may be laminated or placed in a sheet protector to increase durability. Affixed labels as well as multiple sheets of paper in a single sheet protector are prohibited.
Calculators: Two (2) stand-alone non-programmable, non-graphing calculators (Class II), including scientific calculators (e.g. TI-36) are allowed.
Time: 50 minutes
Tiebreakers: 5, 6, 11, 14, 20, 22, 24, 29, 35, 40
Answer the following multiple choice and short answer questions. All multiple choice questions are worth 1 point and short answer questions vary in point amounts.
What determines the optimal annealing temperature for PCR?
Use the image above to answer questions 4-6
Which model of DNA replication is shown by model (a)?
Which model of DNA replication is shown by model (b)?
Which model of DNA replication is shown by model (c)?
Which event does NOT occur in Meiosis?
If a strand of DNA has 18% Cytosine, what percentage Thymine does the strand have?
What start codon initiates translation?
At what temperature does the first step of PCR occur?
Which of the following cell types are haploid (n)?
In the cell cycle, interphase can be divided into which of the following stages?
Which of the following steps of M phase does NOT occur in animal cells but occurs in plant cells?
Cancerous cells do NOT have which of the following?
Sally is worried she has a genetic disorder and decides to get genetic testing done. After analyzing her karyotype, she notices she only has 1 X chromosome! What can you tell Sally to accurately describe her condition?
A single diploid germ cell with n = 18 chromosomes undergoes meiosis. After telophase I and the first cytokinesis during meiosis, what is the state of the cells?
What are SSB’s and how are they used in transcription?
Which of the following attaches to chromosomal centromeres in metaphase?
The coding strand is TTCCGATATACG (5’ to 3’ direction). What might the mRNA synthesized during transcription be?
Which of the following scenarios does not follow the normal flow of genetic information as described by the Central Dogma?
An enveloped virus has a genome with the following characteristics: positive-sense, single-stranded with no mRNA production. Which of the following can be inferred about the virus?
What direction is DNA synthesized and what type of bonds form?
A mother has type A blood and the father has type B blood. What are possible blood types for their offspring?
Which of the following correctly describes the central dogma?
A DNA strand with sequence 5’ CGA TTG 3’ would be complementary to the sequence:
What does PCR stand for?
The structure of coiled DNA and proteins that forms in a cell’s nucleus prior to mitosis is a
What type of polymerase is used in PCR and why?
The X-linked recessive trait of color-blindness is present in 5% of males. If a mother who is a carrier and father who is unaffected plan to have 2 children, what is the probability the children will both be male and color-blind?
What did Hershey and Chase conclude from their experiments with bacteriophages?
George is working with eukaryotic cells in his lab and is observing cell division. He notices that after each round of replication, the chromosomes in the cells are getting shorter and shorter! Which enzymes could have a mutation that makes it nonfunctional based on this information?
The following image pertains to questions 36-38
What type of image is shown above?
Based on the image above, what sex is this individual?
Based on the image above, what genetic disease does this individual have?
Which feature of the Watson–Crick model of DNA structure explains its ability to function in replication and gene expression?
In DNA replication, how do the leading and lagging strands differ?
A fictional organism called the Sparkle Clam can exhibit either a smooth, or ribbed outer shell. Having a ribbed outer shell is dominant to having a smooth outer shell (Ribbed shell type expressed by R). The Sparkle Clam also can produce either a pink or white pearl, with the white pearl being the dominant trait (White pearl expressed by W). Which genotypes could express the cross between a smooth & pink pearl Sparkle Clam, and a ribbed & white pearl Sparkle Clam?
A fictional organism called the Sparkle Clam can exhibit either a smooth, or ribbed outer shell. Having a ribbed outer shell is dominant to having a smooth outer shell (Ribbed shell type expressed by R). The Sparkle Clam also can produce either a pink or white pearl, with the white pearl being the dominant trait (White pearl expressed by W). Which of the following punnett squares will correctly express the cross between a smooth & pink pearl Sparkle Clam, and a ribbed & white pearl Sparkle Clam as seen in the previous question?
Which of the following states a key difference between chromosomes and chromatin:
In a pedigree, which shape represents females?
Which of the following blanks fit best in this statement: In transcription, ____ recognizes and binds to the ____ on DNA to transcribe part of a gene.
What are the two ribosomal subunits typically called?
Which of the following correctly finishes this statement: A key difference between mitosis and meiosis is that mitosis only occurs in ____ cells, while meiosis produces ____.
Which of the following would PCR be most helpful in?
Identify the flow of information as seen in the central dogma.
Briefly explain the difference between transcription and translation.
Explain the difference between genotype and phenotype
The small invertebrate krill species Thysanoessa inermis is adapted to cold (4 C° ) seawater. Over the past ten years, there has been a gradual increase in the water temperature of the krill’s habitat. A sustained increase in water temperature may ultimately affect the ability of the krill to survive.
One effect of higher temperatures is protein misfolding within cells. Krill have several hsp genes that code for heat-shock proteins (HSPs). These proteins help prevent protein misfolding or help to refold proteins to their normal shapes.
Scientists conducted experiments on T. inermis to detect changes in the expression of hsp genes when the krill were exposed to temperatures above 4°C. An experimental group of krill was maintained in tanks with 4°C seawater and then placed into tanks with 10°C seawater for approximately three hours. The krill were then given a six-hour recovery period in the 4°C seawater tanks. A control group of krill was moved from a tank of 4°C seawater to another tank of 4°C seawater for approximately three hours and then returned to the original tank. The scientists analyzed hsp gene expression by measuring the concentrations of three mRNAs (I, II, III) transcribed from certain hsp genes in both the heat-shocked krill (Figure 1) and the control krill. For the control krill, no transcription of the hsp genes was detected throughout the test period (data not shown).
Figure 1. Average concentration of three mRNAs (I, II, III) transcribed from hsp genes in krill heat shocked at 10°C. Error bars represent ±2SEx.
Identify the hsp mRNA that has the slowest rate of concentration increase in response to heat-shock treatment.
Describe the trend in the average concentration of mRNA I throughout the experiment.
The scientists hypothesized that the heat-shock protein (HSP) translated from mRNA I plays a greater role in refolding proteins than does the HSP translated from mRNA II. Use the data to support the hypothesis.
mRNAs I and II are transcribed from the same gene. Explain how a cell can produce two different mRNAs from the same gene.