Skip to main content

BirdSO MiniSO Invitational — Food Science

Food Science · Division B · BirdSO MiniSO Invitational · 2021

50 questions · 79.5 points · 50 min· 6 questions from the original omitted

Original test source

Welcome to Food Science! Please read each of the statements below carefully!

You will be given a total of 50 minutes to complete the exam.

Before the exam begins, please make sure that you ONLY have materials that are permitted by the rule.

For this test, you do NOT need to submit your work. As long as you provide the correct final answer value, you will receive full credits. However, if you would like partial credits for Free-Response Questions, you may type in your work.

The test is three sections: (1) Multiple Choice Questions (2) Free-Response Questions and (3) True or False Questions

There will be 15 Multiple Choice Questions, 4 Long Free-Response Questions, and 10 True or False Questions.

*Some Multiple-Choice Questions may have more than one answer. These "Multi-Select Questions" will be indicated with SELECT ALL THAT APPLY.

For multi-select questions, partial credits will be awarded.

Final Note: This exam may be extensive and difficult, but please try your best! Believe in yourself and your partner. Good luck!

Section 1: Multiple Choice Questions (15 Questions)

PassageFor questions 1–3

The following modified excerpt is based from Food, Fermentation, and Micro-organism by Charles W. Bamforth and David J. Cook

The fresh cucumbers are immersed in brine in bulk tanks. The control parameters are pH, temperature, and the level of salt. The brine's pH is modified and controlled with either vinegar or lactic acid. This facilitates the loss of carbon dioxide. During fermentation, the brine is purged with either nitrogen or air. Whereas air is the cheaper option, nitrogen is preferable. Potassium sorbate (0.035%) is typically added to inhibit the growth of fungi. It is crucial that the end product should possess a firm, crisp texture.

Question 1

2 pts

What is the purpose of purging the brine solution with air or nitrogen gas?

Question 2

1 pt

As stated in the question, the three main control parameters are (1) pH (2) temperature, and (3) salinity level. These factors are modified to make sure that the environment of the vegetable is suitable for fermentation. Which of the following combination provides an appropriate condition for the cucumber fermentation process?

Question 3

2 pts

Although not mentioned in the text above, the fermentation of the cucumber relies on natural organisms. One of the most commonly seen organisms during the fermentation of cucumber is Lactobacillus Plantarum. Which of the following is TRUE regarding the bacteria?

PassageFor questions 4–6

Even after the fermented food is made, it is extremely important to control which types of microorganisms are present. This is to make sure that no individual becomes sick after consuming the product. To control or inhibit the growth of unnecessary organisms, food scientists depend on chemical agents. These chemical agents range from substances that are naturally produced during the fermentation to those that are prepared separately.

Question 4

1.5 pts · select all that apply

Which of the following is(are) relevant factor(s) when deciding what method/agents to use for controlling the growth of microorganisms? SELECT ALL THAT APPLY

Question 5

1.5 pts · select all that apply

Nisin is an antibacterial peptide that contains 34 amino acid residues. It belongs to the Class I bacteriocins. Unlike many other bacteriocins, nisin can cover a very broad spectrum that is effective against many gram-positive bacteria. Thus, it is widely used as a chemical agent to preserve foods. Based on this information, determine which of the following are correct. SELECT ALL THAT APPLY.

Question 6

1.5 pts · select all that apply

Which of the following is/are appropriate method(s) that can be used on foods to control or inhibit the growth of microorganisms? SELECT ALL THAT APPLY.

FigureFor questions 9–11

Consider the following diagram for Questions 8 to 10.

Question 9

1.5 pts · select all that apply

Which of the sugar(s) shown above is (are) categorized as a reducing sugar? SELECT ALL THAT APPLY.

Question 10

1.5 pts

Which of the sugar shown above is the basic starting material that allows the production of pyruvate during glycolysis?

Question 11

1 pt

Based on the diagram shown, which of the following statements is TRUE?

FigureFor questions 12–15

Enzymes are essential biological molecules that facilitate the metabolism processes within an organism. With more than 1000 different types, enzymes catalyze many important reactions that allow organisms to live. Without enzymes, the human's metabolic pathway may be disturbed.

Question 12

1 pt

TRUE OR FALSE All enzymes are protein. (T/F)

Question 13

1 pt

Enzymes are extremely sensitive to their surroundings, so factors such as temperature can heavily impact its behavior. Which of the following does not impact the enzyme's activity or its reaction rate?

Question 14

0.5 pts

What quantity should be placed on the vertical axis on the graph shown?

Question 15

0.5 pts

What quantity should be placed on the horizontal axis on the graph shown?

Section 2: Free Response Questions.

PassageFor questions 1a, 1b, 1c, 1e

Question 1: Water Activity

Water is an important part of food and is the most significant factor when it comes to food safety. Its amount can change how many chemical and biochemical processes occur within the food, which can modify its conditions. Thus, in order to understand how to safely preserve foods, understanding the concept of water activity is important. Through this question, we will explore the fundamentals of water activity.

Question 1a

1.5 pts

To understand the concept of water activity, we must first understand the properties of water first. State at least three characteristics of water molecules.

Question 1b

4 pts

When we discuss "water activity", many students confuse that term with "water content." However, there is a clear distinction between those two. Water content is not a reliable predictor when it comes to deciding how safe the food product will be while water activity is. Describe the difference between those two terms and explain why water content is not a reliable factor when it comes to determining the safety of a product.

Question 1c

2 pts

State the formula for the water activity and interpret what each variable represents.

Question 1e

1 pt

Please describe TWO ways that food scientists can use to reduce the of a food product.

FigureFor questions 2a, 2b, 2c, 2d-i, 2d-ii, 2e-i, 2e-ii, 2e-iii, 2f-i, 2f-ii

Question 2: Sauerkraut Fermentation

Sauerkraut is a fermented cabbage that is believed to be originated from China (the name can be quite deceiving!) This food is well known for being a "superfood" and for having a great taste. The steps of creating Sauerkraut can be seen below.

Question 2a

1 pt

What is the main purpose of adding salt?

Question 2b

1 pt

As seen on the diagram, the salt concentration in sauerkraut is usually within 0.7 to 2.5% (w/w). Why is this the case?

Question 2c

4 pts

The second option is based on the process called spontaneous fermentation. This process depends on the naturally occurring Lactic acid bacteria on the vegetable itself, which is quite low in cabbages. Although this is the traditional method, there are many risks related to spontaneous fermentation. Give at least one reason why spontaneously fermented sauerkraut may be risky to consume.

Question 2d-i

1 pt

As mentioned before, the first option (adding the starter culture) is more likely to produce a product that is safe for consumption compared to the second option. What is a starter culture?

Question 2d-ii

3 pts

As mentioned before, the first option (adding the starter culture) is more likely to produce a product that is safe for consumption compared to the second option.

Why would the addition of a starter culture lead to a safer food product?

Question 2e-i

2 pts

As the fermentation process begins, different types of lactic acid bacteria start to grow within the cabbage. In the beginning, heterofermentative lactic acid bacteria dominate the microflora. However, as time passes, the heterofermentative acid bacteria are replaced by homofermentative lactic acid bacteria. What are the roles of the lactic acid bacteria throughout the fermentation process?

Question 2e-ii

2 pts

As the fermentation process begins, different types of lactic acid bacteria start to grow within the cabbage. In the beginning, heterofermentative lactic acid bacteria dominate the microflora. However, as time passes, the heterofermentative acid bacteria are replaced by homofermentative lactic acid bacteria.

What is(are) the difference(s) between the heterofermentative and homofermentative lactic acid bacteria?

Question 2e-iii

3 pts

As the fermentation process begins, different types of lactic acid bacteria start to grow within the cabbage. In the beginning, heterofermentative lactic acid bacteria dominate the microflora. However, as time passes, the heterofermentative acid bacteria are replaced by homofermentative lactic acid bacteria.

The diagram below shows both heterofermentative and homofermentative processes. Fill in the labels.

Question 2f-i

1 pt

With the conclusion of the fermentation, the Sauerkraut now undergoes the packaging process. Please fill in the sentence blanks below. The choice of packaging material depends largely on whether it would be ____ treated or stored as ____.

Question 2f-ii

1 pt

When would glass or metal containers be preferred for storage over plastic containers?

PassageFor questions 3a, 3b, 3c, 3e, 3f, 3g-i, 3g-ii, 3g-iii

Question 3: Citric Acid Cycle and Fermentation Reactions.

Citric acid cycle and fermentation reactions are a few of the many chemical reactions that occur within many living organisms. While they are distinct processes, each uses similar molecules and has similar purposes.

Question 3a

1 pt

Let's start with the citric acid cycle. Where does this reaction occur? (Be specific as possible)

Question 3b

8 pts

The citric acid cycle begins when the Acetyl-Coa from the oxidized pyruvate attaches to the oxaloacetate. Fill in the diagram shown below and describe each process as one structure is changed to the next one. (Your description should include what is being added/lost and how the structure is changing) Start with structure A. (Each description should not be longer than three sentences. Try to keep it simple and to the point!)

Question 3c

1 pt

How many ATPs will be produced given that two molecules of glucose will be used to produce pyruvate?

Question 3e

2.5 pts

The diagram shows that GTP molecules are formed by substrate-level phosphorylation. What is the difference between GTP and ATP in terms of its component?

Question 3f

3 pts

As you can see in the diagram, the citric acid cycle does not directly use oxygen. However, in the absence of oxygen the process soon shuts down. Explain why this is the case.

Question 3g-i

2 pts

State the products that alcohol fermentation produces and the products that lactic acid fermentation produces.

Question 3g-ii

1 pt

Provide an example of a food that is produced through alcohol fermentation and another that is produced through lactic acid fermentation.

Question 3g-iii

2 pts

Describe the difference in steps between the two fermentation processes after the glucose has been converted to pyruvate through glycolysis.

PassageFor questions 4a, 4b, 4c, 4d, 4e

Question 4: Nutrition Labeling Regulations

Nutrition labeling regulations are crucial for standardizing an outline that properly provides the necessary information to the consumers. Answer the following questions regarding the regulations. (1.00 point each)

Question 4a

1 pt

Where should the label be placed on the food products that are stored inside containers and packages?

Question 4b

1 pt

What information MUST appear on the PDP?

Question 4c

1 pt

Where should the Nutrition facts label be placed on the container of the food package?

Question 4d

1 pt

The FDA requires what two vitamins to be declared on the Nutrition Facts label?

Question 4e

1 pt

How is the total fat of the food product determined when it comes to Nutrition facts labeling?

Section 3: True or False Questions.

Let's end the test with some easy questions :D! Please write T or F for each statement below. Each question is worth 1 point.

Question 3-1

1 pt

Dill pickle is an example of an acidified food.

Question 3-2

1 pt

An acidified food has an equilibrium pH level of 4.6 or less and a water activity of lower than 0.85.

Question 3-3

1 pt

Yeast's scientific name is Saccharomyces cerevisiae

Question 3-4

1 pt

On the nutrition label, potassium should be listed directly under sodium

Question 3-5

1 pt

Water is not used to destroy the peptide bonds in sucrose.

Question 3-6

1 pt

Fructose is an example of aldose sugar.

Question 3-7

1 pt

Citrate is the ionized form of citric acid.

Question 3-8

1 pt

Substrate level phosphorylation occurs when a phosphate group of a substrate is directly transferred to ADP.

Question 3-9

1 pt

Chocolate is not an example of fermented food.

Question 3-10

1 pt

In cellular respiration, substrate-level phosphorylation produces the majority of the ATP.